CST3 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Cystatin C; CST3; CST3 elisa kit;
The Human CST3 cst3 (Catalog #MBS355465) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS355465 ELISA Kit recognizes Human CST3. MyBioSource\'s Cystatin C can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA).For quantitative detection of Cystain C in human serum, plasma, body fluids, tissue lysates or cell culture supernatants. Researchers should empirically determine the suitability of the CST3 cst3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CST3 cst3 product has the following accession number(s) (GI #755738) (NCBI Accession #CAA29096.1) (Uniprot Accession #P01034). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Cystatin C Human ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Cystatin C polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Cystatin C polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Cystatin C amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Cystatin C can be calculated.
Background: Cystatin C or cystatin 3, a protein encoded by the CST3 gene, is mainly used as a biomarker of kidney function. It is a single 120-residue polypeptide with a molecular mass of approximately 13.26 kD. Cystatin C plays a role in network reorganization in the epileptic dentate gyrus, especially in granule cell dispersion and guidance of migrating newborn granule cells. It is an alternative measure of kidney function that may have prognostic importance among elderly persons who do not meet standard criteria for chronic kidney disease. Among elderly persons without chronic kidney disease, cystatin C is a prognostic biomarker of risk for death, cardiovascular disease, and chronic kidney disease, thereby signaling a state of \'preclinical\' kidney dysfunction.
Assay Type: Sandwich
Detection Range: 312 pg/ml-20,000 pg/ml
Sensitivity: < 10 pg/ml.