CST3 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Cystatin C; CST3; CST3 elisa kit;
The Human CST3 cst3 (Catalog #MBS175874) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS175874 ELISA Kit recognizes Human CST3.The CST3 cst3 product has the following accession number(s) (GI #118183) (NCBI Accession #P01034.1) (Uniprot Accession #P01034). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Cystatin C PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) (Human Cystatin C PicoKine ELISA Kit standard curve )
Principle of the assay: human Cystatin C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cystatin C has been precoated onto 96-well plates. Standards(NSO, M1-A146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cystatin C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cystatin C amount of sample captured in plate.
Background: Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons),and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the mostimportant extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by aspecific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4.
Samples: Cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk
Assay Type: Sandwich
Detection Range: 312pg/ml-20,000pg/ml
Sensitivity: < 10pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.