CST elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; C+B137Atestatin; CST elisa kit; CST;
The Mouse CST n/a (Catalog #MBS744582) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS744582 ELISA Kit recognizes Mouse CST.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse C+B137atestatin ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This CST ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CST. This ELISA kit for research use only!
Principle of the Assay: CST ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CST antibody and an CST-HRP conjugate. The assay sample and buffer are incubated together with CST-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CST concentration since CST from samples and CST-HRP conjugate compete for the anti-CST antibody binding site. Since the number of sites is limited, as more sites are occupied by CST from the sample, fewer sites are left to bind CST-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CST concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Mouse C+B137atestatin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.