|The Rat Crhr1 crhr1 (Catalog #MBS760058) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS760058 ELISA Kit recognizes Rat (Rattus, General) Crhr1.
The Crhr1 crhr1 product has the following accession number(s) (GI #223717971) (NCBI Accession #NP_001138618.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Corticotropin-releasing factor receptor 1 ELISA Kit with the following immunoassay(s):
Typical Testing Data
Typical Standard Curve
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Crhr1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Crhr1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Crhr1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Crhr1 can be calculated.
Samples: Serum, plasma and other biological fluids.
Assay Type: Sandwich ELISA, Double Antibody
Detection Range: 0.312-20ng/ml
Sensitivity: 0.188ng/ml. Rat Corticotropin-releasing factor receptor 1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Crhr1 also interacts with the following gene(s): CREB1, CRH, CRHBP, DLG1, POMC, SLC2A1, UCN, UCN2, YY1. Brain, Embryonic Tissue, Eye, Ovary tissues are correlated with this protein.