CP elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; CP elisa kit; Creatine Phosphate;
The Mouse CP n/a (Catalog #MBS746275) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS746275 ELISA Kit recognizes Mouse CP.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Creatine Phosphate ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This COX7A2L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCOX7A2L. This ELISA kit for research use only!
Principle of the Assay: COX7A2L ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COX7A2L antibody and an COX7A2L-HRP conjugate. The assay sample and buffer are incubated together with COX7A2L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX7A2L concentration since COX7A2L from samples and COX7A2L-HRP conjugate compete for the anti-COX7A2L antibody binding site. Since the number of sites is limited, as more sites are occupied by COX7A2L from the sample, fewer sites are left to bind COX7A2L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX7A2L concentration in each sample is interpolated from this standard curve.
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type: Quantitative Competitive
Sensitivity: 0.1 ng/mL. Mouse Creatine Phosphate ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.