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Cotinine elisa kit product blog

Posted on 2016-02-01 14:17:10 by mybiosource_staff
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Tags: ELISA Kit; Rat ELISA Kit; Cotinine; COTININE elisa kit;
The Rat Cotinine n/a (Catalog #MBS728205) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS728205 ELISA Kit recognizes Rat (Rattus, General) Cotinine.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Cotinine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
 Typical Testing Data/Standard Curve (for reference only) Cotinine.

Intended Uses: This COTN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat COTN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: COTN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COTN antibody and an COTN-HRP conjugate. The assay sample and buffer are incubated together with COTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COTN concentration since COTN from samples and COTN-HRP conjugate compete for the anti-COTN antibody binding site. Since the number of sites is limited, as more sites are occupied by COTN from the sample, fewer sites are left to bind COTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COTN concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 ng/mL. Rat Cotinine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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