Cortisol elisa kit product blog
Tags: ELISA Kit; Cortisol; Cortisol elisa kit;
The Cortisol n/a (Catalog #MBS580005) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: Competitive immunoenzymatic colorimetric method for the determination of Estriol in saliva. For research use only.
Principle of the Assay: The Estriol (antigen) in the sample competes with the antigenic Estriol conjugated with horseradish peroxidase (HRP) for binding to the limited number of antibodies anti Estriol coated on the microplate (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. Then, the enzyme HRP in the bound-fraction reacts with the Substrate (H2O2) and the TMB Substrate and develops a blu color that changes into yellow when the Stop Solution is added. The colour intensity is inversely proportional to the Estriol concentration in the sample. Estriol concentration in the sample is calculated through a calibration curve
Background/Introduction: Estriol (also Oestriol) is one of the three main estrogens produced by the human body. It is only produced in significant amounts during pregnancy as it is made by the fetus. During pregnancy the production of estriol depends on an intact maternal-placental-fetal unit. Fetal-placental production of estriol leads to a progressive rise in maternal circulating levels reaching a late-gestational peak several orders of magnitude greater than non-pregnant levels. In the maternal circulation, estriol undergoes a rapid conjugation in the liver followed by urinary excretion with a half-life of about 20 minutes. Since normal estriol production depends on an intact maternal-placental-fetal circulation and functional fetal metabolism, maternal estriol levels have been used to monitor fetal status during pregnancy, particularly during the third trimester. DHEA-S is produced by the adrenal cortex of the fetus, this is converted to estriol by the placenta. If levels are abnormally low in a pregnant woman, this may indicate a problem with the development in the child. Levels of estriol in non-pregnant women do not change much after menopause, and levels are not significantly different from levels in men.
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Intra-assay Precision: Within run variation was determined by replicate (16x) the measurement of two different saliva control in one assay. The within assay variability is <= 9.7%
Inter-assay Precision: Between run variation was determined by replicate (10x) the measurement of two different saliva control with different lots of kit. The between assay variability is <= 13.7%. Cortisol ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.