Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow CoQ10 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • DUTP elisa kit blog
 • TGF-b1 clia kit blog
 • HSD17B10 elisa kit blog
 • ACTG1 elisa kit blog
 • OB elisa kit blog
 • IL-4 elisa kit blog
 • GP210 elisa kit blog
 • GHRH elisa kit blog
 • GROG elisa kit blog
 • UNC13D elisa kit blog
 • DEFB114 elisa kit blog
 • ACSM5 elisa kit blog
 • FRA elisa kit blog
 • RELA elisa kit blog
 • ADD1 elisa kit blog
 • P2RY2 elisa kit blog
 • CLEC9A elisa kit blog
 • GLI1 elisa kit blog
 • ADAD1 elisa kit blog
 • MRPL11 elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Angiotensin-converting enzyme (ACE) Recombinant Protein
 • Microsomal Triglyceride Transfer Protein (MTTP) ELISA Kit
 • Gzms-B ELISA Kit
 • squamous cell carcinoma antigen recognized by T cells 3 (SA... Antibody
 • Progesterone Antibody
 • Integrin alpha-L (Itgal) Recombinant Protein
 • 17-beta-hydroxysteroid dehydrogenase type 6 (HSD17B6) ELISA Kit
 • Transglutaminase (TG) ELISA Kit
 • carbonic anhydrase 2, CA-2 ELISA Kit
 • DDI2 Antibody
 • 25-Hydroxycholesterol (25-OHC) ELISA Kit
 • PRDX4 Recombinant Protein
 • Lipase, Lipoprotein (LIPD) ELISA Kit
 • Extracellular serine-threonine protein kinase FAM20C (FAM20C... Recombinant Protein
 downarrow more ...

CoQ10 elisa kit product blog

Posted on 2017-03-04 02:36:56 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Mouse ELISA Kit; Coenzyme Q10 (CoQ10); CoQ10 elisa kit; CoQ10;
The Mouse CoQ10 n/a (Catalog #MBS7233016) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7233016 ELISA Kit recognizes Mouse (Murine, General) CoQ10.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Coenzyme Q10 (CoQ10) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
 Typical Testing Data/Standard Curve (for reference only) CoQ10.

Intended Uses: This CQ10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CQ10. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CQ10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CQ10 antibody and an CQ10-HRP conjugate. The assay sample and buffer are incubated together with CQ10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CQ10 concentration since CQ10 from samples and CQ10-HRP conjugate compete for the anti-CQ10 antibody binding site. Since the number of sites is limited, as more sites are occupied by CQ10 from the sample, fewer sites are left to bind CQ10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CQ10 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 ng/mL. Mouse Coenzyme Q10 (CoQ10) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions