Complement C1c+C1q elisa kit product blog
Tags: ELISA Kit; Complement C1c+C1q; Complement C1c+C1q elisa kit;
The Complement C1c+C1q n/a (Catalog #MBS539749) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Complement C1c+C1q can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA).Highly purified human C1q is bound to microwells. Antibodies aginst this antigen, if present in diluted serum, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue colour. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample. Researchers should empirically determine the suitability of the Complement C1c+C1q n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
ELISA kit for detection of Complement C1c and C1q in the research laboratory.
Biological Significance: The presence of Anti-C1q autoantibodies is associated with several autoimmune and renal illnesses. Containing an occurrence of 100% in the hypocomplementaemic uricarial vasculitis syndrome (HUVS), Anti-C1q autoantibodies act as a marker for this disease. They were also described in systemic lupus erythemathodes (SLE) and especially in lupus nephritis.
Biohazard Information: All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV I/II, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal. Complement C1c+C1q ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.