|The Mouse COL10 n/a (Catalog #MBS2530675) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2530675 CLIA Kit recognizes Mouse (Murine, General) COL10.
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for complete product instructions).
Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Mouse COL10 (Collagen Type X) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of COL10 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to COL10. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for COL10 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain COL10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of COL10. You can calculate the concentration of COL10 in the samples by comparing the RLU value of the samples to the standard curve.
Assay Type: Sandwich
Detection Range: 62.5-4000pg/mL
Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL.