COL10 clia kit product blog
Tags: COL10; CLIA Kit; Collagen Type X (COL10); Human CLIA Kit; COL10 clia kit;
The Human COL10 n/a (Catalog #MBS2000442) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2000442 CLIA Kit recognizes Human COL10.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Human Collagen Type X (COL10) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of AMH in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to AMH. A competitive inhibition reaction is launched between biotin labeled AMH and unlabeled AMH (Standards or samples) with the pre-coated antibody specific to AMH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of AMH in the sample. Then the mixture of substrate An and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the AMH level in the sample or standard.
Samples: Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Assay Type: Quantitative Competitive
Detection Range: 61.73-5,000pg/mL
Sensitivity: 22.84pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AMH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AMH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%.