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Col II elisa kit product blog

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Posted on 2015-10-12 22:56:13 by mybiosource_staff
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Tags: ELISA Kit; Mouse ELISA Kit; Collagen Type II, Col II; Col II elisa kit; Col II ;
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The Mouse Col II n/a (Catalog #MBS703307) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS703307 ELISA Kit recognizes Mouse (Murine, General) Col II.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Collagen Type II, Col II ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) Col II.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Col II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Col II present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Col II is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Col II bound in the initial step. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma, tissue homogenates
Assay Type: Sandwich
Detection Range: 1.87 ng/ml -120 ng/ml.
Sensitivity: The minimum detectable dose of mouse Col II is typically less than 0.46 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Mouse Collagen Type II, Col II ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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