Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow CK 18 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • GPCR elisa kit blog
 • CNR-1 elisa kit blog
 • MFRP elisa kit blog
 • RAD50 elisa kit blog
 • SEPT5 elisa kit blog
 • MED21 elisa kit blog
 • GPR78 elisa kit blog
 • OAS1 elisa kit blog
 • OR5H14 elisa kit blog
 • COX6A2 elisa kit blog
 • PPP3R1 elisa kit blog
 • PPID elisa kit blog
 • MSR1 elisa kit blog
 • SFAS/APO-1 elisa kit blog
 • gammaEP elisa kit blog
 • BMF elisa kit blog
 • DEF elisa kit blog
 • ACD elisa kit blog
 • FBLN3 elisa kit blog
 • NCAPH2 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • glutamic acid decarboxylase Antibody (GAD-Ab) ELISA Kit
 • PfLDH Antibody
 • CD8b (Ly 3) (CD8b (Ly 3)) Antibody
 • Interferon Alpha - Beta Receptor (IFN-alpha - betaR) ELISA Kit
 • R-Spondin 1 (RSPO1) ELISA Kit
 • Annexin II (ANN-II) ELISA Kit
 • NOL10 Antibody
 • WAVE1 (N-terminal region) Peptide (WASF1) Peptide
 • IgA (IGHA1) Purified Protein
 • ADAM17 Antibody
 • G protein-coupled receptor kinase 5 (GRK5) ELISA Kit
 • B-Cell Leukemia - Lymphoma 2 (Bcl-2) ELISA Kit
 • Anthrax Protective Antigen IgG (APA IgG) ELISA Kit
 • FAA Antibody
 downarrow more ...
separator

CK 18 elisa kit product blog

separator
Posted on 2016-04-14 16:40:19 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; Cytokeratin 18; CK 18 elisa kit; CK 18;
separator
The Human CK 18 krt18 (Catalog #MBS733383) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS733383 ELISA Kit recognizes Human (General) CK 18.

The CK 18 krt18 product has the following accession number(s) (GI #34037) (NCBI Accession #CAA31375.1) (Uniprot Accession #P05783). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Intended Uses: This CK18 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CK18. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CK18 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CK18. Standards or samples are then added to the microtiter plate wells and CK18 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CK18 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CK18 are added to each well to "sandwich" the CK18 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CK18 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CK18 concentration in each sample is interpolated from this standard curve.

CK 18 krt18

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive or Sandwich
Detection Range: 0.5-10ng/mL
Sensitivity: 0.1ng/mL. Human CytoKeratin 18 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Adenocarcinoma, Breast Neoplasms, Carcinoma, Fatty Liver, Fibrosis, Liver Cirrhosis, Liver Diseases, Liver Neoplasms, Necrosis, Neoplasm Metastasis are some of the diseases may be linked to Human CytoKeratin 18 ELISA Kit. CK 18 also interacts with the following gene(s): CASP6, KRT8, TCHP.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions