Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow CII elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • UEA elisa kit blog
 • CLDN14 elisa kit blog
 • PRB3 elisa kit blog
 • GSTa1 elisa kit blog
 • SARM1 elisa kit blog
 • COL1A1 elisa kit blog
 • SLC25A2 elisa kit blog
 • APELIN elisa kit blog
 • TTC21B elisa kit blog
 • CD244 elisa kit blog
 • P-GP elisa kit blog
 • NPIP elisa kit blog
 • GAL14 elisa kit blog
 • NAV2 elisa kit blog
 • INHA elisa kit blog
 • INSL5 elisa kit blog
 • TLS elisa kit blog
 • BCR elisa kit blog
 • FGF-18 elisa kit blog
 • MOG elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Transaldolase (TALDO1) Antibody
 • Aromatic L-Amino Acid Decarboxylase, DOPA Decarboxylase, DDC Antibody
 • adiponectin, C1Q and collagen domain containing (ADIPOQ) ELISA Kit
 • alpha-Glucosidase ELISA Kit
 • Cy3 Conjugated Avidin Secondary Antibody
 • Parathyroid hormone-related protein (PTHLH) ELISA Kit
 • Thromboglobulin, beta (bTG) ELISA Kit
 • Glial fibrillary acidic protein - GFAP Antibody
 • PNLIPRP1 Antibody
 • Butyrylcholinesterase (BCHE) Antibody
 • CHRM4 - M4 Antibody
 • ADAMTS4 Antibody
 • sema domain, immunoglobulin domain (Ig) , transmembrane doma... ELISA Kit
 • fibroblast growth factor 23 (FGF23) ELISA Kit
 downarrow more ...
separator

CII elisa kit product blog

separator
Posted on 2015-11-02 13:51:41 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Goat ELISA Kit; Anti-Type II Collagen Antibody; CII; CII elisa kit;
separator
The Goat CII col2a1 (Catalog #MBS751061) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS751061 ELISA Kit recognizes Goat (Caprine, General) CII.

The CII col2a1 product has the following accession number(s) (GI #180809) (NCBI Accession #AAA52037.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Goat Anti-Type II Collagen Antibody ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) CII.

Intended Uses: This Anti-CII ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat Anti-CII. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: Anti-CII ELISA kit applies the competitive enzyme immunoassay technique utilizing Type II Collagen antigen and an Anti-CII-HRP conjugate. The assay sample and buffer are incubated together with Anti-CII-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-CII concentration since Anti-CII from samples and Anti-CII-HRP conjugate compete for the Type II Collagen antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-CII from the sample, fewer sites are left to bind Anti-CII-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-CII concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Goat Anti-Type II Collagen Antibody ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Eye, Heart, Kidney, Muscle, Skin tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions