Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit assay kit product blog
Tags: Assay Kit; Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit; Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit assay kit;
The Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit n/a (Catalog #MBS841830) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Choline / Acetylcholine Quantification Colorimetric / Fluorometric Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Choline/Acetylcholine Quantification Kit: Colorimetric & Fluorometric Assay to Measure Choline and Acetylcholine in various Biological Samples such as Blood, Tissue, Cells, Culture Media, Fermentation media, etc.within 40 min. Simple, Sensitive & Accurate. Detection Range: 10 pmol-5 nmol. 100 Assays
Background: Choline and acetylcholine play important roles in many biological processes. MyBioSource\'s Choline/Acetylcholine Quantification Kit provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method. In the assay free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (lambda= 570 nm), and fluorescence (Ex/Em 535/587 nm). Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction. The kit can detect choline and acetylcholine (total choline - free choline) in various biological samples such as in blood, cells, culture media, fermentation media, etc. There is no need for pretreatment or purification of samples. The kit can detect 10 pmol~5 nmol of choline or acetylcholine.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Application- The kit can detect 10 pmol to ~5 nmol of choline or acetylcholine.
Samples: Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids. Features & Benefits: Simple procedure; takes ~40 minutes
Fast and convenient
Kit provides all necessary buffers and reagents for an accurate assay of Choline/Acetylcholine in various biological samples.