|The Human Chlamydia Trachomatis IgA n/a (Catalog #MBS494611) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494611 ELISA Kit recognizes Human (General) Chlamydia Trachomatis IgA.
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Intended use The Chlamydia Trachomatis IgA ELISA kit is intended for the detection of IgA antibody to Chlamydia Trachomatis in human serum or plasma. For research use only, not for use in diagnostic procedures.
Principle of the test Diluted serum (serum diluent contains sorbent to remove rheumatoid factor and human IgG interference) is added to wells coated with purified antigen. IgA specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgA specific antibody in the unknown.
Chlamydia Trachomatis IgA ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.