Chlamydia pneumoniae antibody (Cpn IgM) elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Chlamydia pneumoniae antibody (Cpn IgM); Chlamydia pneumoniae antibody (Cpn IgM) elisa kit;
The Human Chlamydia pneumoniae antibody (Cpn IgM) n/a (Catalog #MBS7207815) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7207815 ELISA Kit recognizes Human Chlamydia pneumoniae antibody (Cpn IgM).To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Chlamydia pneumoniae antibody (Cpn IgM) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This CPn IgM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CPn IgM. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: CPn IgM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CPn IgM antibody and an CPn IgM-HRP conjugate. The assay sample and buffer are incubated together with CPn IgM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPn IgM concentration since CPn IgM from samples and CPn IgM-HRP conjugate compete for the anti-CPn IgM antibody binding site. Since the number of sites is limited, as more sites are occupied by CPn IgM from the sample, fewer sites are left to bind CPn IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPn IgM concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 ug/mL. Human Chlamydia pneumoniae antibody (Cpn IgM) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.