CHGA elisa kit product blog
Tags: ELISA Kit; Horse ELISA Kit; CHGA elisa kit; CHGA; Chromogranin-A;
The Horse CHGA chga (Catalog #MBS280439) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS280439 ELISA Kit recognizes Horse CHGA.The CHGA chga product has the following accession number(s) (GI #823683845) (NCBI Accession #NP_001075283.2) (Uniprot Accession #Q9XS63). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: For the quantitative detection of Horse Chromogranin-A (CHGA) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CHGA in Horse serum, plasma. An antibody specific for CHGA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHGA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHGA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHGA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 1.56 ng/mL - 100 ng/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%. Horse Chromogranin-A (CHGA) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. CHGA also interacts with the following gene(s): GAST, SCG2, SCG3, SYP. Carcinoma, Non-Small-Cell Lung, Cardiovascular Diseases, Diabetes Mellitus, Hyperplasia, Hypertension, Inflammation, Kidney Diseases, Liver Diseases, Nervous System Diseases, Pheochromocytoma are some of the diseases may be linked to Horse Chromogranin-A (CHGA) ELISA Kit.