CER elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; CER elisa kit; CER; Ceramide;
The Human CER n/a (Catalog #MBS7254089) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7254089 ELISA Kit recognizes Human CER.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Ceramide ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) ()
Intended Uses: This CER ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CER. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: CER ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CER antibody and an CER-HRP conjugate. The assay sample and buffer are incubated together with CER-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CER concentration since CER from samples and CER-HRP conjugate compete for the anti-CER antibody binding site. Since the number of sites is limited, as more sites are occupied by CER from the sample, fewer sites are left to bind CER-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CER concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 ng/mL. Human Ceramide ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.