CD36 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; CD36; CD36 elisa kit;
The Human CD36 cd36 (Catalog #MBS824511) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS824511 ELISA Kit recognizes Human CD36. MyBioSource\'s CD36 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (SE). Researchers should empirically determine the suitability of the CD36 cd36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The CD36 cd36 product has the following accession number(s) (GI #48375178) (NCBI Accession #NP_000063.2) (Uniprot Accession #P16671). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human CD36 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The MyBioSource\' Human CD36 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD36 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD36 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD36 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD36 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD36 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.
Samples: Cell Culture Supernatants, Serum, Plasma
Assay Type: Quantitative Sandwich
Sensitivity: <10 pg/ml. Human CD36 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Adipose Tissue, Blood, Bone, Bone Marrow, Brain, Connective Tissue, Heart, Liver, Muscle, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. Atherosclerosis, Cardiovascular Diseases, Dyslipidemias, Fatty Liver, Heart Diseases, Inflammation, Insulin Resistance, Liver Diseases, Malaria, Necrosis are some of the diseases may be linked to Human CD36 ELISA Kit.