|The Human CCL14 ccl14 (Catalog #MBS912846) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS912846 ELISA Kit recognizes Human (General) CCL14.
The CCL14 ccl14 product has the following accession number(s) (GI #14589959) (NCBI Accession #NP_116738.1) (Uniprot Accession #Q16627). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL14 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Assay Type: Sandwich. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Human C-C motif chemokine 14, CCL14 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. CCL14 also interacts with the following gene(s): CCR1, CCR3, CCR5. Heart, Skin, Spleen, Thymus, Uterus tissues are correlated with this protein. The following patways have been known to be associated with this gene. Breast Neoplasms, Kidney Diseases, Neoplasm Metastasis, Neoplasms, Experimental, Ovarian Neoplasms are some of the diseases may be linked to Human C-C motif chemokine 14, CCL14 ELISA Kit.