Caspase-5 assay kit product blog
Tags: Assay Kit; Caspase-5; Caspase-5 assay kit;
The Caspase-5 n/a (Catalog #MBS841757) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Product Information: Caspase-5 Activity Colorimetric Assay Kit: Simple & Convenient Colorimetric Assay to Measure Caspase-5 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays.
Background: The Caspase-5 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspase-5 and related caspases that recognize the sequence WEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate WEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
Summary: Detection method- Absorbance (400 or 405 nm)
Species reactivity- Mammalian
Kit size- Convenient sizes (25, 100, 200, 400 assays)
Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Samples: Cell and tissue lysates. Features & Benefits: Simple one-step procedure; takes only 1-2 hours
Fast and convenient
Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-5 activity.