|The Caspase-3 n/a (Catalog #MBS841649) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.
The Caspase-3 n/a product has the following accession number(s) (GI #14790119) (NCBI Accession #NP_004337.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Caspase-3 Colorimetric Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Caspase-3/CPP32 Activity Colorimetric Assay Kit: Simple & Convenient Colorimetric Assay to Measure Caspase-3/CPP32 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays.
Background: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
Summary: Detection method- Absorbance (400 or 405 nm)
Species reactivity- Mammalian
Kit size- Convenient sizes (25, 100, 200, 400 assays)
Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Samples: Cell and tissue lysates. Features & Benefits: Simple one-step procedure; takes 1-2 hours
Fast and convenient
Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.