CASP8 assay kit product blog
Tags: CASP8; Caspase-8; Assay Kit; CASP8 assay kit;
The CASP8 n/a (Catalog #MBS841557) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.The CASP8 n/a product has the following accession number(s) (GI #45382997) (NCBI Accession #NP_989923.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Product Information: Caspase-8/FLICE Activity Colorimetric Assay Kit: Simple & Convenient colorimetric Assay to Measure Caspase-8/FLICE Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays.
Background: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
Summary: Detection method- Absorbance (400 or 405 nm)
Species reactivity- Mammalian
Kit size- Convenient sizes (25, 100, 200, 400 assays)
Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Samples: Cell and tissue lysates. Features & Benefits: Simple one-step procedure; takes 1-2 hours
Fast and convenient
Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity. The following patways have been known to be associated with this gene.