Cardiolipin IgA elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Cardiolipin IgA; Cardiolipin IgA elisa kit;
The Human Cardiolipin IgA n/a (Catalog #MBS494678-ORG 515A) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494678-ORG 515A ELISA Kit recognizes Human Cardiolipin IgA.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Principle of the assay: Highly purified cardiolipin is bound to microwells saturated with beta2-glycoprotein I. Antibodies to these antigens, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated anti-human IgA immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgA antibodies present in the original sample.
Samples: Human serum or plasma
Sensitivity: The lower detection limit for Anti-Cardiolipin IgA was determined at 1.0 APL-U/ml. Intended Uses: Anti-Cardiolipin IgA is an indirect solid phase enzyme immunoassay (ELISA) for the quantitative measurement of IgA class autoantibodies against cardiolipin in human serum or plasma. Anti-Cardiolipin IgA ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.