|The Mouse CAPN10 capn10 (Catalog #MBS2022333) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2022333 ELISA Kit recognizes Mouse (Murine, General) CAPN10.
The CAPN10 capn10 product has the following accession number(s) (GI #37589919) (NCBI Accession #AAH07553.2) (Uniprot Accession #Q9HC96). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Calpain 10 (CAPN10) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CAPN10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CAPN10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CAPN10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CAPN10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Mouse tissue homogenates and other biological fluids
Detection Range: 0.312-20ng/mL
Sensitivity: The minimum detectable dose of mouse CAPN10 is typically less than 0.103ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CAPN10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CAPN10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CAPN10 in mouse tissue homogenates and other biological fluids. Calpain 10 (CAPN10) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Adipose Tissue, Blood, Brain, Heart, Kidney, Liver, Muscle, Pancreas, Prostate, Vascular tissues are correlated with this protein. Carcinoma, Hepatocellular, Congenital Abnormalities, Diabetes Mellitus, Type 2, Disease Models, Animal, Fatty Liver, Hypertension, Liver Diseases, Liver Neoplasms, Necrosis, Nervous System Diseases are some of the diseases may be linked to Calpain 10 (CAPN10) ELISA Kit.