CA125 elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; Carbohydrate Antigen 125; CA125 elisa kit; CA125;
The Rat CA125 n/a (Catalog #MBS732014) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS732014 ELISA Kit recognizes Rat CA125.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Carbohydrate Antigen 125 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This CA125 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CA125. This ELISA kit for research use only!
Principle of the Assay: CA125 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CA125 antibody and an CA125-HRP conjugate. The assay sample and buffer are incubated together with CA125-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CA125 concentration since CA125 from samples and CA125-HRP conjugate compete for the anti-CA125 antibody binding site. Since the number of sites is limited, as more sites are occupied by CA125 from the sample, fewer sites are left to bind CA125-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA125 concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 5.0-100U/mL
Sensitivity: 1.0u/ml. Rat Carbohydrate Antigen 125 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.