Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow Ca (2+) -ATPase elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • FMO3 elisa kit blog
 • Caspase-6 elisa kit blog
 • MLLT3 elisa kit blog
 • ACHRAB elisa kit blog
 • OR5B17 elisa kit blog
 • DLAT elisa kit blog
 • GnIH elisa kit blog
 • PPM1A elisa kit blog
 • CNN3 elisa kit blog
 • CAPN11 elisa kit blog
 • SELL elisa kit blog
 • MMRN2 elisa kit blog
 • STAT5 elisa kit blog
 • GZMS-A elisa kit blog
 • PGS1 elisa kit blog
 • GCA elisa kit blog
 • URRBP elisa kit blog
 • CL elisa kit blog
 • PRO-ANP elisa kit blog
 • KLR elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Influenza B Antibody
 • PNLIP cDNA Clone
 • Plexin-A1 (PLXNA1) Recombinant Protein
 • Bone morphogenetic protein 7 (BMP7) Recombinant Protein
 • Arsenite methyltransferase (AS3MT) ELISA Kit
 • IL-1alpha ELISA Kit
 • Transforming growth factor beta, TGF-beta ELISA Kit
 • Interleukin-18 (IL18) ELISA Kit
 • alpha thalassemia-mental retardation syndrome X-linked (RAD5... ELISA Kit
 • Anti-endothelial cell antibody, AECA ELISA Kit
 • GMEB2 Antibody
 • Retinal dehydrogenase 1 (ALDH1A1) ELISA Kit
 • pLDH Antibody
 • IFN-gamma ELISA Kit
 downarrow more ...
separator

Ca (2+) -ATPase elisa kit product blog

separator
Posted on 2015-11-09 16:40:36 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Mouse ELISA Kit; Ca (2+) -ATPase; Ca (2+) -ATPase elisa kit;
separator
The Mouse Ca (2-) -ATPase n/a (Catalog #MBS728028) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS728028 ELISA Kit recognizes Mouse (Murine, General) Ca (2-) -ATPase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Ca (2+) -ATPase ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) ()
Typical Testing Data/Standard Curve (for reference only) Ca (2-) -ATPase.

Principle of the Assay: Ca2+/ATPase ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Ca2+/ATPase antibody and an Ca2+/ATPase-HRP conjugate. The assay sample and buffer are incubated together with Ca2+/ATPase-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Ca2+/ATPase concentration since Ca2+/ATPase from samples and Ca2+/ATPase-HRP conjugate compete for the anti-Ca2+/ATPase antibody binding site. Since the number of sites is limited, as more sites are occupied by Ca2+/ATPase from the sample, fewer sites are left to bind Ca2+/ATPase-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ca2+/ATPase concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Intended Uses: This Ca2+/ATPase ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Ca2+/ATPase. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Mouse Ca (2+) -ATPase ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions