Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow C4B elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • CRH-R elisa kit blog
 • PDIA4 elisa kit blog
 • CNIH4 elisa kit blog
 • PDGFC elisa kit blog
 • PAR2 elisa kit blog
 • R2AE1 elisa kit blog
 • CH25H elisa kit blog
 • alpha1M elisa kit blog
 • PSRC1 elisa kit blog
 • PRO-HEPC elisa kit blog
 • LIFR elisa kit blog
 • RANTES elisa kit blog
 • anti-cardiolipin antibody IgM, ACA-IgM elisa kit blog
 • OR13F1 elisa kit blog
 • ADAMTSL4 elisa kit blog
 • CFB elisa kit blog
 • SPAST elisa kit blog
 • PCP elisa kit blog
 • CNTD1 elisa kit blog
 • TTPalpha elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • DDX24 Antibody
 • Caspase 9 (Ab-125) (CASP9) Antibody
 • Integrin beta3 (Phospho-Tyr785) (ITGB3) Antibody
 • DUSP22 Antibody
 • Calcitonin Gene Related Peptide (CGRP) ELISA Kit
 • Stromal Cell Derived Factor 1Beta (SDF-1BETA) ELISA Kit
 • Occludin (OCLN) ELISA Kit
 • Terminal Complement Complex C5B-9 (TCC C5B-9) ELISA Kit
 • Costimulatory Molecules B7H4 (B7-H4) ELISA Kit
 • Dihydrolipoyl Transacetylase (DLAT) ELISA Kit
 • Paired Box Gene 9 (PAX9) ELISA Kit
 • Aldosterone ELISA Kit
 • C-Peptide ELISA Kit
 • Myeloid-Related Protein (MRP) ELISA Kit
 downarrow more ...

C4B elisa kit product blog

Posted on 2015-07-22 09:27:11 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Guinea Pig ELISA Kit; C4B; C4b elisa kit; Complement Component 4B;
The Guinea Pig C4B c4b (Catalog #MBS720461) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS720461 ELISA Kit recognizes Guinea Pig (Cavia, Cavy, Porcellus, General) C4B.

The C4B c4b product has the following accession number(s) (GI #356601440) (NCBI Accession #AET24773.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Guinea pig Complement Component 4B ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) C4B.

Principle of the assay: C4B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C4B antibody and an C4B-HRP conjugate. The assay sample and buffer are incubated together with C4B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C4B concentration since C4B from samples and C4B-HRP conjugate compete for the anti-C4B antibody binding site. Since the number of sites is limited, as more sites are occupied by C4B from the sample, fewer sites are left to bind C4B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4B concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 ng/mL. Intended Uses: This C4B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig C4B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Guinea pig Complement Component 4B ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions