Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow C4B elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • APO-C4 elisa kit blog
 • MDH2 elisa kit blog
 • IL-1 elisa kit blog
 • ANG1-7 elisa kit blog
 • CPR elisa kit blog
 • GAD1 elisa kit blog
 • MYL9 elisa kit blog
 • Celf6 elisa kit blog
 • TRMU elisa kit blog
 • TNKS1BP1 elisa kit blog
 • AKR1C4 elisa kit blog
 • HPF4 elisa kit blog
 • CHMP1B elisa kit blog
 • FABP1 elisa kit blog
 • TMC7 elisa kit blog
 • ERBB4 elisa kit blog
 • STK elisa kit blog
 • INHB elisa kit blog
 • KYNURENINE elisa kit blog
 • CCDC92 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • GRM1 Antibody
 • beta-Tubulin Antibody
 • SOD1 Recombinant Protein
 • Retinol dehydrogenase 11 (RDH11) Antibody
 • Regucalcin (RGN) ELISA Kit
 • Renalase (RNLS) Recombinant Protein
 • XRCC4-like factor - NHEJ1 Peptide
 • GFP Antibody
 • RNASE3 Antibody
 • YSK4 Sps1-Ste20-related kinase homolog (S. cerevisiae) ELISA Kit
 • ATP-binding cassette sub-family G member 2 (ABCG2) ELISA Kit
 • Nicastrin (NCSTN) Recombinant Protein
 • Interleukin 35 (IL 35) ELISA Kit
 • secreted protein acidic and rich in cysteine, SPARC ELISA Kit
 downarrow more ...
separator

C4B elisa kit product blog

separator
Posted on 2015-07-22 09:27:11 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Guinea Pig ELISA Kit; C4B; C4b elisa kit; Complement Component 4B;
separator
The Guinea Pig C4B c4b (Catalog #MBS720461) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS720461 ELISA Kit recognizes Guinea Pig (Cavia, Cavy, Porcellus, General) C4B.

The C4B c4b product has the following accession number(s) (GI #356601440) (NCBI Accession #AET24773.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Guinea pig Complement Component 4B ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) C4B.

Principle of the assay: C4B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C4B antibody and an C4B-HRP conjugate. The assay sample and buffer are incubated together with C4B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C4B concentration since C4B from samples and C4B-HRP conjugate compete for the anti-C4B antibody binding site. Since the number of sites is limited, as more sites are occupied by C4B from the sample, fewer sites are left to bind C4B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4B concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 50-1000ng/mL
Sensitivity: 1.0ng/mL. Intended Uses: This C4B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig C4B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Guinea pig Complement Component 4B ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions