C3bR elisa kit product blog
Tags: ELISA Kit; Chicken ELISA Kit; C3bR elisa kit; C3bR; Complement Fragment 3B Rccptor;
The Chicken C3bR n/a (Catalog #MBS736312) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS736312 ELISA Kit recognizes Chicken C3bR.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Chicken Complement fragment 3b rccptor ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: C3bR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C3bR antibody and an C3bR-HRP conjugate. The assay sample and buffer are incubated together with C3bR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C3bR concentration since C3bR from samples and C3bR-HRP conjugate compete for the anti-C3bR antibody binding site. Since the number of sites is limited, as more sites are occupied by C3bR from the sample, fewer sites are left to bind C3bR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3bR concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Intended Uses: This C3bR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken C3bR. This ELISA kit for research use only, not for therapeutic applications!. Chicken Complement fragment 3b rccptor ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.