Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow C3 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • NEF3 elisa kit blog
 • DAP6 elisa kit blog
 • hnRNPAb elisa kit blog
 • SFT elisa kit blog
 • HAVCR1 elisa kit blog
 • CAT elisa kit blog
 • OPM elisa kit blog
 • SNAP-25 elisa kit blog
 • GPT2 elisa kit blog
 • ATGL elisa kit blog
 • AAMP elisa kit blog
 • FABP7 elisa kit blog
 • SCD86 elisa kit blog
 • CXCR2 elisa kit blog
 • PKB elisa kit blog
 • KISS1 elisa kit blog
 • CHRNA1 elisa kit blog
 • Erbb3 elisa kit blog
 • CHST9 elisa kit blog
 • F9 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • HLA G Antibody
 • CCAAT-enhancer binding protein(C-EBP) Alpha (CEBP-a) Recombinant Protein
 • Jumonji Domain Containing 2A (KDM4A) Antibody
 • Anti-IgA Antibody ELISA Kit
 • S100 Calcium Binding Protein A9 (S100A9) ELISA Kit
 • cathepsin B (CTSB) ELISA Kit
 • Somatostatin (SST) ELISA Kit
 • SOD Assay Kit
 • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (M... ELISA Kit
 • Cysteine-rich protein 2 (P52943) Recombinant Protein
 • Aquaporin-1 (AQP1) ELISA Kit
 • Nuclease P1 Enzyme
 • AANAT cDNA Clone
 • MitoPT JC-1 Assay Kit
 downarrow more ...
separator

C3 elisa kit product blog

separator
Posted on 2018-06-13 04:09:49 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Rat ELISA Kit; Complement Component 3; C3 elisa kit;
separator
The Rat C3 c3 (Catalog #MBS760493) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS760493 ELISA Kit recognizes Rat (Rattus, General) C3.

The C3 c3 product has the following accession number(s) (GI #152012784) (NCBI Accession #AAI50180.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Complement Component 3 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
 Typical Testing Data/Standard Curve (for reference only) C3.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- C3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- C3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C3 can be calculated.

Samples: serum, plasma, tissue homogenates and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 3.125-200ng/ml
Sensitivity: <1.875ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Rat Complement Component 3 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Anemia, Brain Diseases, Cardiovascular Diseases, Hemorrhage, Inflammation, Kidney Diseases, Liver Diseases, Lung Diseases, Necrosis, Proteinuria are some of the diseases may be linked to Rat Complement Component 3 ELISA Kit. The following patways have been known to be associated with this gene. Blood, Bone, Brain, Kidney, Liver, Lung, Muscle, Nerve, Skin, Spinal Cord tissues are correlated with this protein. C3 also interacts with the following gene(s): CD46, CFD, CFH, CFHR1, CFI, CFP, CR1, CR2, ITGAM, VSIG4.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions