Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow C3 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • TMEM168 elisa kit blog
 • SLC39A6 elisa kit blog
 • GROA elisa kit blog
 • cortisone elisa kit blog
 • SMC2 elisa kit blog
 • GABA elisa kit blog
 • FDX1L elisa kit blog
 • U S-GH elisa kit blog
 • PENTOSIDINE elisa kit blog
 • MMP-12 elisa kit blog
 • EOMES elisa kit blog
 • HE-4 elisa kit blog
 • SCGB2A2 elisa kit blog
 • IFN-beta elisa kit blog
 • TXA2 elisa kit blog
 • APF elisa kit blog
 • GRAP elisa kit blog
 • LCE1A elisa kit blog
 • TREM-1 clia kit blog
 • FOXL2 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Versican (VCAN) ELISA Kit
 • Vascular Endothelial Growth Factor D (VEGF.D) ELISA Kit
 • brain-derived neurotrophic factor (BDNF) ELISA Kit
 • IP6K2 Antibody
 • Procollagen I N-Terminal Propeptide (PINP) ELISA Kit
 • Fibroblast growth factor 23 (FGF23) ELISA Kit
 • Anti-Biotin, HRP Antibody
 • Bacteroides thetaiotaomicron Antibody
 • Prostate Specific Antigen (PSA) ELISA Kit
 • IgM ELISA Kit
 • Pancreatic hormone (PPY) Recombinant Protein
 • Prostaglandin E1 (PGE1) ELISA Kit
 • Treponema pallidum Antibody
 • ARSB - Arylsulfatase B Antibody
 downarrow more ...
separator

C3 elisa kit product blog

separator
Posted on 2015-10-07 22:10:22 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Bovine ELISA Kit; Complement Component 3; C3 elisa kit;
separator
The Bovine C3 c3 (Catalog #MBS735174) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS735174 ELISA Kit recognizes Bovine (Cow, Cattle, General) C3.

The C3 c3 product has the following accession number(s) (GI #40786791) (NCBI Accession #AAR89906.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine Complement Component 3 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) ()
Typical Testing Data/Standard Curve (for reference only) C3.

Principle of the Assay: C3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C3 antibody and an C3-HRP conjugate. The assay sample and buffer are incubated together with C3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C3 concentration since C3 from samples and C3-HRP conjugate compete for the anti-C3 antibody binding site. Since the number of sites is limited, as more sites are occupied by C3 from the sample, fewer sites are left to bind C3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 250-5000ng/ml
Sensitivity: 1.0ng/ml. Intended Uses: This C3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine C3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Bovine Complement Component 3 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Bone, Brain, Kidney, Liver, Lung, Muscle, Nerve, Skin, Spinal Cord tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions