C3 elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; C3 elisa kit; Complement 3, C3;
The Rat C3 n/a (Catalog #MBS703000) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS703000 ELISA Kit recognizes Rat C3.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Complement 3, C3 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with C3. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for C3. The competitive inhibition reaction is launched between with pre-coated C3 and C3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of C3 in the samples. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates
Assay Type: Competitive
Detection Range: 0.625 ug/ml-40 ug/ml
Sensitivity: 0.156 ug/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Rat Complement 3, C3 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.