Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow C1q elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • LDHA elisa kit blog
 • PLAC8 elisa kit blog
 • PROCR elisa kit blog
 • AAA elisa kit blog
 • MDH1 elisa kit blog
 • POLgamma1 elisa kit blog
 • FIL1d elisa kit blog
 • PI3K2A elisa kit blog
 • CX3CL1 elisa kit blog
 • TUBA1C elisa kit blog
 • RPL36A elisa kit blog
 • H2AFZ elisa kit blog
 • GSTO1 elisa kit blog
 • OR52B6 elisa kit blog
 • HABP elisa kit blog
 • ICAM-1/CD54 elisa kit blog
 • FABP5 elisa kit blog
 • FBXO21 elisa kit blog
 • SPARCL1 elisa kit blog
 • MPO-ANCA IgG elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • leucyl-tRNA synthetase 2 (LARS2) Antibody
 • COG8 Antibody
 • CD42b Antibody
 • Leptin, carboxyl terminal (residues 131-145) Antibody
 • Interleukin-2 Receptor Alpha (IL-2RA) (IL-2RA ) Recombinant Protein
 • LPP Peptide
 • Newcastle Disease Virus Antibody
 • Proteasome 19S, Subunit S5A (RPN10) Antibody
 • CD99 Antibody
 • ARPC2 Antibody
 • Matrix protein 2 (M2) Recombinant Protein
 • NKX2-8 Antibody
 • Heparanase 2 - HPSE2 Antibody
 • PRKAA2 Peptide
 downarrow more ...
separator

C1q elisa kit product blog

separator
Posted on 2016-04-04 12:51:47 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Porcine ELISA Kit; Complement 1Q; C1Q elisa kit; C1Q;
separator
The Porcine C1q c1qtnf5 (Catalog #MBS737182) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS737182 ELISA Kit recognizes Porcine (Pig, Swine, General) C1q.

The C1q c1qtnf5 product has the following accession number(s) (GI #14149712) (NCBI Accession #NP_056460.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Porcine Complement 1q ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) C1q.

Principle of the assay: C1q ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C1q antibody and an C1q-HRP conjugate. The assay sample and buffer are incubated together with C1q-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C1q concentration since C1q from samples and C1q-HRP conjugate compete for the anti-C1q antibody binding site. Since the number of sites is limited, as more sites are occupied by C1q from the sample, fewer sites are left to bind C1q-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1q concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Intended Uses: This C1q ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine C1q. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Porcine Complement 1q ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain, Connective Tissue, Eye, Heart, Muscle, Ovary, Placenta, Skin, Vascular tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions