|The Bovine BP180-Ab col17a1 (Catalog #MBS750460) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS750460 ELISA Kit recognizes Bovine (Cow, Cattle, General) BP180-Ab.
The BP180-Ab col17a1 product has the following accession number(s) (GI #1877435) (NCBI Accession #AAB51499.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine Anti-bullous pemphigoid 180 antibody ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This BP180-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine BP180-Ab.
Principle of the Assay: BP180-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Bullous Pemphigoid 180 antigen and an BP180-Ab-HRP conjugate. The assay sample and buffer are incubated together with BP180-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BP180-Ab concentration since BP180-Ab from samples and BP180-Ab-HRP conjugate compete for the Bullous Pemphigoid 180 antigen binding site. Since the number of sites is limited, as more sites are occupied by BP180-Ab from the sample, fewer sites are left to bind BP180-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BP180-Ab concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 ng/mL. Bovine Anti-bullous pemphigoid 180 antibody ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. BP180-Ab also interacts with the following gene(s): COL7A1, DST, ITGB4, LAMB3, LAMC2, PLEC.