Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow BP180-Ab elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • AICDA elisa kit blog
 • AQP-2 elisa kit blog
 • NFIL3 elisa kit blog
 • Lrp8 elisa kit blog
 • FBG elisa kit blog
 • GHR/GHBP elisa kit blog
 • HAVlgG elisa kit blog
 • Arid3a elisa kit blog
 • TTLL10 elisa kit blog
 • ZSWIM1 elisa kit blog
 • MAC elisa kit blog
 • 6-K-PG elisa kit blog
 • CLEC2L elisa kit blog
 • ASH1L elisa kit blog
 • APOC2 elisa kit blog
 • CCSA-2 elisa kit blog
 • C5aR1 elisa kit blog
 • BANP elisa kit blog
 • UACA elisa kit blog
 • ATF6 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • PLA2G2D ELISA Kit
 • ZAK Antibody
 • Procollagen Type III N-Terminal Propeptide (P3NP) ELISA Kit
 • Interleukin-5 (rIL 5) Recombinant Protein
 • Chloride channel protein 2 (CLCN2) Recombinant Protein
 • OASIS (CREB3L1) Antibody
 • Catalase Assay Kit
 • FLVCR2 Antibody
 • PGAM1-PGAM2-PGAM4 Antibody
 • Albumin Antibody
 • Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14... Antibody
 • Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) ELISA Kit
 • Fibrinogen Degradation Product (FDP) ELISA Kit
 • Ang-II ELISA Kit
 downarrow more ...
separator

BP180-Ab elisa kit product blog

separator
Posted on 2017-03-04 01:58:24 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Bovine ELISA Kit; BP180-Ab elisa kit; BP180-Ab; Anti-bullous pemphigoid 180 antibody;
separator
The Bovine BP180-Ab col17a1 (Catalog #MBS750460) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS750460 ELISA Kit recognizes Bovine (Cow, Cattle, General) BP180-Ab.

The BP180-Ab col17a1 product has the following accession number(s) (GI #1877435) (NCBI Accession #AAB51499.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine Anti-bullous pemphigoid 180 antibody ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) BP180-Ab.

Intended Uses: This BP180-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine BP180-Ab.

Principle of the Assay: BP180-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Bullous Pemphigoid 180 antigen and an BP180-Ab-HRP conjugate. The assay sample and buffer are incubated together with BP180-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BP180-Ab concentration since BP180-Ab from samples and BP180-Ab-HRP conjugate compete for the Bullous Pemphigoid 180 antigen binding site. Since the number of sites is limited, as more sites are occupied by BP180-Ab from the sample, fewer sites are left to bind BP180-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BP180-Ab concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 ng/mL. Bovine Anti-bullous pemphigoid 180 antibody ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. BP180-Ab also interacts with the following gene(s): COL7A1, DST, ITGB4, LAMB3, LAMC2, PLEC.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions