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bMSH elisa kit product blog

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Posted on 2015-11-11 02:59:29 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; Beta-Melanocyte Stimulating Hormone (bMSH); bMSH elisa kit; bMSH;
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The Human bMSH n/a (Catalog #MBS2024758) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2024758 ELISA Kit recognizes Human (General) bMSH.

The bMSH n/a product has the following accession number(s) (GI #1168040) (NCBI Accession #AAB35187.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Beta-Melanocyte Stimulating Hormone (bMSH) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) bMSH.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Melanocyte Stimulating Hormone (bMSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Melanocyte Stimulating Hormone (bMSH) and unlabeled Beta-Melanocyte Stimulating Hormone (bMSH) (Standards or samples) with the pre-coated antibody specific to Beta-Melanocyte Stimulating Hormone (bMSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Melanocyte Stimulating Hormone (bMSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Melanocyte Stimulating Hormone (bMSH) in the sample.

Assay Type: Competitive Inhibition
Samples: Serum, Plasma and Other Biological Fluids
Detection Range: 37.04-3000pg/mL
Sensitivity: < 15.3pg/mL. Application: Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Melanocyte Stimulating Hormone (bMSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Melanocyte Stimulating Hormone (bMSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =: SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%. Beta-Melanocyte Stimulating Hormone (bMSH) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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