|The Human BMG/beta2-MG n/a (Catalog #MBS2516203) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2516203 ELISA Kit recognizes Human (General) BMG/beta2-MG.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human BMG/beta2-MG (Beta-2-Microglobulin) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human BMG/beta2-MG concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with BMG/beta2-MG. During the reaction, BMG/beta2-MG in the sample or standard competes with a fixed amount of BMG/beta2-MG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BMG/beta2-MG. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of BMG/beta2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 31.25-2000ng/mL
Sensitivity: 18.75ng/mL. Human BMG/beta2-MG (Beta-2-Microglobulin) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.