Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow BK elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • TSGF elisa kit blog
 • GJA5 elisa kit blog
 • CSN2 elisa kit blog
 • ADM elisa kit blog
 • RAB7A elisa kit blog
 • HIS elisa kit blog
 • PTP elisa kit blog
 • SOX3 elisa kit blog
 • IL2Rbeta1 elisa kit blog
 • CA-IgA elisa kit blog
 • ANGPTL4 elisa kit blog
 • CSTB elisa kit blog
 • PROM1 elisa kit blog
 • MC5R elisa kit blog
 • LACRT elisa kit blog
 • GCNT3 elisa kit blog
 • ACP2 elisa kit blog
 • DNAH11 elisa kit blog
 • SMM elisa kit blog
 • DPP4 assay kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • EDNRB Antibody
 • Band 3 Antibody
 • Heat Shock Protein 70 (HSP70) Antibody
 • Interleukin-8 (IL-8) Western Blot Control
 • Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit
 • Caspase-9 (CASP9) Antibody
 • Free Triiodothyronine (fT3) ELISA Kit
 • (Rhesus macaque) C-C chemokine receptor type 5 (CCR5) Recombinant Protein
 • C Reactive Protein (CRP) Recombinant Protein
 • FBXL4 Antibody
 • Polyadenylate-binding protein RBP47A (RBP47A) Recombinant Protein
 • c-Met Antibody
 • Total beta Amyloid Protein (Abeta) ELISA Kit
 downarrow more ...

BK elisa kit product blog

Posted on 2015-08-05 21:37:55 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Monkey ELISA Kit; Bradykinin; BK elisa kit;
The Monkey BK n/a (Catalog #MBS739901) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS739901 ELISA Kit recognizes Monkey (Rhesus Macaque, Rhesus Monkey, General) BK.

The BK n/a product has the following accession number(s) (GI #15593992) (NCBI Accession #CAC69834.1) (Uniprot Accession #P83060). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Monkey Bradykinin ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) BK.

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This BK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey BK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY BK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BK antibody and an BK-HRP conjugate. The assay sample and buffer are incubated together with BK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BK concentration since BK from samples and BK-HRP conjugate compete for the anti-BK antibody binding site. Since the number of sites is limited, as more sites are occupied by BK from the sample, fewer sites are left to bind BK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BK concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 ng/mL. Monkey Bradykinin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions