|The beta 2-Glycoprotein (ApoH) n/a (Catalog #MBS564182) is an ELISA Kit produced from Human and is intended for research purposes only. The product is available for immediate purchase.
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Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the B2G present in samples reacts with the anti-B2G antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-B2G antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound B2G. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3\',5,5\'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of B2G in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of B2G in the test sample. The quantity of B2G in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Samples: Immunoperoxidase Assay for Determination of Beta 2-Glycoprotein in Human Samples
Assay Type: Sandwich. Intended Uses: The Beta 2-Glycoprotein (B2G) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring B2G in biological fluids of Human. Human beta 2-Glycoprotein (ApoH) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.