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BDF elisa kit product blog

Posted on 2016-01-18 13:14:06 by mybiosource_staff
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Tags: ELISA Kit; Canine ELISA Kit; beta Defensins; BDF elisa kit; BDF;
The Canine BDF n/a (Catalog #MBS743658) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS743658 ELISA Kit recognizes Canine (Dog, General) BDF.

The BDF n/a product has the following accession number(s) (Uniprot Accession #Q863C9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Canine beta Defensins ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) ()
Typical Testing Data/Standard Curve (for reference only) BDF.

Principle of the Assay: BDF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BDF antibody and an BDF-HRP conjugate. The assay sample and buffer are incubated together with BDF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BDF concentration since BDF from samples and BDF-HRP conjugate compete for the anti-BDF antibody binding site. Since the number of sites is limited, as more sites are occupied by BDF from the sample, fewer sites are left to bind BDF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDF concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Intended Uses: This BDF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine BDF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Canine beta Defensins ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Metabolic Diseases are some of the diseases may be linked to Canine beta Defensins ELISA Kit. BDF also interacts with the following gene(s): CAMP, DEFB104A.
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