|The Horse BCHE bche (Catalog #MBS933821) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS933821 ELISA Kit recognizes Horse (Equine, General) BCHE.
The BCHE bche product has the following accession number(s) (GI #21362409) (NCBI Accession #P81908.1) (Uniprot Accession #P81908). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Horse Cholinesterase, BCHE ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with BCHE. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for BCHE. The competitive inhibition reaction is launched between with pre-coated BCHE and BCHE in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of BCHE in the samples. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma and tissue homogenates.
Assay Type: Competitive
Detection Range: 5 mU/ml-80 mU/ml.
Sensitivity: 2.5 mU/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Horse Cholinesterase, BCHE ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. BCHE also interacts with the following gene(s): ALB, APP, CHAT, COLQ, GHRL, INS, PRIMA1. The following patways have been known to be associated with this gene.