|The BCA Protein Assay Kit II n/a (Catalog #MBS841734) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.
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Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the BCA Protein Assay Kit II with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: BCA Protein Assay Kit II: Detergent compatible protein quantitation kit. linear over a wide range of protein concentrations between 25-2000 mug/ml. Includes BSA. 2500, 5000 assays
Background: MyBioSource\'s BCA Protein Assay kit is useful for colorimetric detection and quantification of total protein content even in the presence of detergents. The Kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in an alkaline medium. The amount of purple-colored product formed during the reaction can be correlated to the amount of protein in the assay. The Cu+1-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a wide range of protein concentrations between 25-2000 mug/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard. This BCA Protein Assay Kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of the samples.
Summary: Detection method- Absorbance (562 nm)
Features & Benefits: Simple & convenient
High linearity: linear over a wide range of protein concentrations between 25-2000 mug/ml.