|The General Bb n/a (Catalog #MBS2515860) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2515860 ELISA Kit recognizes General (Zebra) Bb.
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Intended Uses: This ELISA kit applies to the invitro quantitative determination of Bbconcentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Bb. During the reaction,Bb in the sample or standard competes with a fixed amount ofBb on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Bb. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration ofBb in the samples is then determined by comparing the OD of the samples to the standard curve.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 0.313-20ug/mL
Sensitivity: 0.188ug/mL. Bb(Bilirubin) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.