|The Rat BACE1 bace1 (Catalog #MBS705548) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS705548 ELISA Kit recognizes Rat (Rattus, General) BACE1.
The BACE1 bace1 product has the following accession number(s) (GI #9506421) (NCBI Accession #NP_062077.1) (Uniprot Accession #P56819). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat beta-site APP-Cleaving Enzyme 1, BACE1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BACE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BACE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BACE1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BACE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates
Assay Type: Sandwich
Detection Range: 0.625 ng/ml-40 ng/ml.
Sensitivity: The minimum detectable dose of rat BACE1 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Rat beta-site APP-Cleaving Enzyme 1, BACE1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Brain, Ear, Embryonic Tissue, Kidney, Liver, Muscle, Ovary, Pancreas, Vascular tissues are correlated with this protein. BACE1 also interacts with the following gene(s): Adam10, Aplp1, App, Ddit3, Gga1, Gga3, Jak2, Psen1, Rtn3, Srebf2. The following patways have been known to be associated with this gene. Alzheimer Disease, Brain Diseases, Brain Injuries, Cognition Disorders, Inflammation, Learning Disorders, Memory Disorders, Necrosis, Nerve Degeneration, Pain are some of the diseases may be linked to Rat beta-site APP-Cleaving Enzyme 1, BACE1 ELISA Kit.