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ATP5I elisa kit product blog

Posted on 2016-03-05 07:22:44 by mybiosource_staff
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Tags: ELISA Kit; Rat ELISA Kit; ATP5I; ATP synthase subunit e, mitochondrial (ATP5I); ATP5I elisa kit;
The Rat ATP5I atp5i (Catalog #MBS7209472) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7209472 ELISA Kit recognizes Rat (Rattus, General) ATP5I.

The ATP5I atp5i product has the following accession number(s) (GI #6005717) (NCBI Accession #NP_009031.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat ATP synthase subunit e, mitochondrial (ATP5I) ELISA Kit with the following immunoassay(s):
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data ATP5I.

Intended Uses: This ATP5I ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ATP5I. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ATP5I ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATP5I antibody and an ATP5I-HRP conjugate. The assay sample and buffer are incubated together with ATP5I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP5I concentration since ATP5I from samples and ATP5I-HRP conjugate compete for the anti-ATP5I antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP5I from the sample, fewer sites are left to bind ATP5I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP5I concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 100-2500pg/mL
Sensitivity: 1.0pg/ml. Rat ATP synthase subunit e, mitochondrial (ATP5I) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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