AT3G55810 assay kit product blog
Tags: Pyruvate Kinase; Assay Kit; AT3G55810 assay kit; AT3G55810;
The AT3G55810 f1i16_220 (Catalog #MBS841626) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.The AT3G55810 f1i16_220 product has the following accession number(s) (GI #15228196) (NCBI Accession #NP_191140.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Pyruvate Kinase Activity Colorimetric / Fluorometric Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Pyruvate Kinase Activity Assay Kit: Colorimetric & Fluorometric Assay for Measuring Pyruvate Kinase Activity in various Biological Samples such as Blood, Tissues, Cultured cells etc. within 30 min. Convenient, Accurate & Reliable. Detection Limit: 0.1 mU. 100 Assays.
Background: Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. MyBioSource provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at lambda = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The kit detects 0.1 mU/ml pyruvate kinase.
Samples: Purified/Isolated enzyme, Testing inhibitor panels. Features & Benefits: Simple procedure; takes ~ less than 40 minutes
Fast and convenient. The following patways have been known to be associated with this gene.