|The Mouse AST esm1 (Catalog #MBS281715) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS281715 ELISA Kit recognizes Mouse (Murine, General) AST.
The AST esm1 product has the following accession number(s) (GI #208022668) (NCBI Accession #NP_001129076.1) (Uniprot Accession #Q9NQ30). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Endothelial cell-specific molecule 1 is encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. The cDNA sequence contains an open reading frame of 552 nucleotides. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue is restricted to the lung. Immunoblotting and immunoprecipitation identified a 20-kD protein.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ESM1 in samples. An antibody specific for ESM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyESM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ESM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ESM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Detection Wavelength: 450 nm. Mouse Aspartate Aminotransferase (AST) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.