Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow ASAH2B elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • LEP elisa kit blog
 • MIgamma elisa kit blog
 • FN elisa kit blog
 • OR1I1 elisa kit blog
 • CKMT1B elisa kit blog
 • AP3B2 elisa kit blog
 • RNLS elisa kit blog
 • MN elisa kit blog
 • AKAP1 elisa kit blog
 • A1CF elisa kit blog
 • DIAPH1 elisa kit blog
 • BFGF 4 elisa kit blog
 • HNF1a elisa kit blog
 • AMBP elisa kit blog
 • CYP4X1 elisa kit blog
 • HSP-70 elisa kit blog
 • UPP2 elisa kit blog
 • PARP4 elisa kit blog
 • DR-70TM elisa kit blog
 • EL elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • DnaK Antibody
 • Myeloid cell surface antigen CD33 (CD33) Recombinant Protein
 • Trimethylguanosine synthase (TGS1) Antibody
 • angiopoietin 2 (ANGPT2) ELISA Kit
 • chemokine (C-C motif) ligand 7 (CCL7) ELISA Kit
 • Magainin 2 (LOC397766) Peptide
 • Haptoglobin (Hpt) ELISA Kit
 • anti-Rat IgM Antibody
 • Medium wave sensitive opsin 1 (OPN1MW) ELISA Kit
 • Tetrahydrofolic Acid (THFA) ELISA Kit
 • Glucagon, GC ELISA Kit
 • oxidized high density lipoprotein (Ox-HDL) ELISA Kit
 • Salmonella pig Antibody ELISA Kit
 • Chondroitin Sulfate (CS) ELISA Kit
 downarrow more ...
separator

ASAH2B elisa kit product blog

separator
Posted on 2017-12-14 12:28:16 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; ASAH2B; Putative inactive neutral ceramidase B; ASAH2B elisa kit;
separator
The Human ASAH2B asah2b (Catalog #MBS280417) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS280417 ELISA Kit recognizes Human (General) ASAH2B.

The ASAH2B asah2b product has the following accession number(s) (GI #118442845) (NCBI Accession #NP_001072984.1) (Uniprot Accession #P0C7U1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ASAH2B in samples. An antibody specific for ASAH2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyASAH2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ASAH2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ASAH2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ASAH2B asah2b

Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Human Putative inactive neutral ceramidase B (ASAH2B) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions