ARG1 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; ARG1; Arginase-1; Arg1 elisa kit;
The Human ARG1 arg1 (Catalog #MBS284037) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS284037 ELISA Kit recognizes Human ARG1.The ARG1 arg1 product has the following accession number(s) (GI #10947139) (NCBI Accession #NP_000036.2) (Uniprot Accession #P05089). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: For the quantitative detection of Human Arginase-1 (ARG1) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ARG1 in Human serum, plasma. An antibody specific for ARG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ARG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 1.56 ng/mL - 100 ng/mL
Sensitivity: 0.52 ng/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%. Human Arginase-1 (ARG1) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Blood, Bone Marrow, Brain, Eye, Liver, Lung, Muscle, Skin, Spleen, Uterus tissues are correlated with this protein. Asthma, Cardiovascular Diseases, Disease Models, Animal, Immune System Diseases, Inflammation, Lung Neoplasms, Necrosis, Neoplasms, Nervous System Diseases, Pain are some of the diseases may be linked to Human Arginase-1 (ARG1) ELISA Kit. ARG1 also interacts with the following gene(s): ARG2, NOS1.