apoTf clia kit product blog
Tags: APOTF; CLIA Kit; Human CLIA Kit; Apotransferrin (apoTf); apoTf clia kit;
The Human apoTf n/a (Catalog #MBS2025261) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2025261 CLIA Kit recognizes Human apoTf.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Human Apotransferrin (apoTf) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
The microplate provided in this kit has been pre-coated with an antibody specific to Apotransferrin (apoTf). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apotransferrin (apoTf). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate An and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apotransferrin (apoTf) level in the sample or standard.;.
Assay Type: Double-antibody Sandwich
Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range: 27.4-20,000pg/mL
Sensitivity: < 10.6pg/mL. Application: Chemiluminescent immunoassay for Antigen Detection.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apotransferrin (apoTf) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apotransferrin (apoTf) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =: SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%.